5 Simple Statements About thr77 Explained
5 Simple Statements About thr77 Explained
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts just isn't yet readily available.
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Blocking peptides are peptides that bind particularly to your goal antibody and block antibody binding. These peptide ordinarily is made up of the epitope recognized through the antibody.
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This guidebook outlines more feasible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to be sure the absolute best outcomes are...
Antibodies bound to the blocking peptide now not bind towards the epitope about the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is unique.
Target Information Cyclin E belongs to the highly conserved cyclin family, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation patterns which contribute for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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